RESOURCES

Kecia has been capable of troubleshooting nearly every RT-qPCR issue that has ever come up in the lab. With more than 20 years of experience, she has tips and tricks that we don't usually think about. She gave lab meeting starting from the basics of DNA/RNA structure and graduating to best ways to interpret melt curves. Here is her presentation. Pay attention to the notes section in the powerpoint as well.

Gillian is known as the supremely organized person in our lab. We asked her to share her tips and tricks for how to stay on top of all things science, and she created this powerpoint. It has terrific suggestions for all of us, and underscores how much of success in science is about having a coherent system to keep track of myriad competing demands.

While the powerpoint is geared towards the Leica system, many of the principles apply to any confocal system. Also in here is an overview of deconvolution principles and using the Stellaris SP8 FALCON microscope.